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Fig. 5 HQD restored the expression levels of BMP2, <t>BMP7,</t> and BMPR2. A.B. Western blot analysis of renal cortical BMP2, BMP7, and BMPR2 expression levels. Values are mean ± SEM (n = 3) *P < 0.05 vs. db/m, #P < 0.05, ##P < 0.01 vs. db/db by one-way ANOVA with Tukey’s post doc analysis
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Fig. 5 HQD restored the expression levels of BMP2, BMP7, and BMPR2. A.B. Western blot analysis of renal cortical BMP2, BMP7, and BMPR2 expression levels. Values are mean ± SEM (n = 3) *P < 0.05 vs. db/m, #P < 0.05, ##P < 0.01 vs. db/db by one-way ANOVA with Tukey’s post doc analysis

Journal: BMC complementary medicine and therapies

Article Title: Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway.

doi: 10.1186/s12906-023-04029-1

Figure Lengend Snippet: Fig. 5 HQD restored the expression levels of BMP2, BMP7, and BMPR2. A.B. Western blot analysis of renal cortical BMP2, BMP7, and BMPR2 expression levels. Values are mean ± SEM (n = 3) *P < 0.05 vs. db/m, #P < 0.05, ##P < 0.01 vs. db/db by one-way ANOVA with Tukey’s post doc analysis

Article Snippet: Then, the membranes were immunoblotted with primary antibodies against BMP2 (Abcam, Ab214821, 1:1000 dilution), BMP7 (Proteintech, 12,221–1-AP, 1:1000 dilution), BMPR2 (Proteintech, 14,376–1-AP, 1:1000 dilution), phospho-ERK (CST, 4370S, 1:2000 dilution), ERK (CST, 4695S, 1:1000 dilution), P-SMAD1,5,9 (CST, 13,820, 1:1000 dilution), SMAD4 (Affinity, AF5247, 1:500 dilution), SMAD1 (CST, 6944, 1:1000 dilution), CXCL16 (Affinity, DF13312, 1:500 dilution) and ß-actin (Abcam, ab6276, 1:10,000 dilution) after blocking in 5% bovine serum albumin in TBST for 1 h. All primary antibodies were incubated at 4 °C overnight.

Techniques: Expressing, Western Blot